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mechanical stress analysis of transfected CHO suspension celles - magLev versus perestaltic pumps_cover

Mechanical Stress Analysis of Transfected CHO Suspension Cells – MagLev versus Peristaltic Pumps

Keywords:
  • Bioprocessing
  • Pump Comparison
  • Peristaltic Pumps
  • Shear Stress
  • Cell Viability

Facts
In comparison to a Levitronix® pump, peristaltic pumps generate flow through tube compression along a pump casing. Tube compression can generate shear stress, which results in reduced cell viability.

Test conditions
One Levitronix® and two peristaltic pumps’ shear stress generation were compared by observing their effect on respective cell viabilities. 3.4 liters of CHO cells were introduced and pumped in three identical closed-loop configurations. The flow rate of each pump was set to 3.4 lpm and different backpressures for a period of over 400 minutes. The cell viability was measured at selected times and compared.

Results
The cell viability with the Levitronix® pump system was consistently above 95%. The peristaltic pumps reached cell viabilities of 70% and 28%, respectively. Similar results have been recorded at four different pressure levels up to 0.5 bar.

time-dependent-viability-of-the-cell-suspension-pumped-with-3.4Lmin-without-back-pressure

Time dependent viability of the cell suspension pumped with 3.4Lmin without back pressure

 

Author Stephan Kaiser, Dieter Eibl
Company ZHAW
Document Number -
Pages 32